Loading spreadsheet…
Opening in Numbers for Mac...
Your browser isn’t fully supported.
For the best Numbers for iCloud experience, use a supported browser.
Learn More
Cancel
Continue
Taq DNA Polymerase
Mastermix Calculator
M
Setup
Mastermix
_
||
X
Made by: Federico Mussso - 2024
No of tubes
(include controls)
16 tubes
Reaction Volume
10,0 uL/tube
DNA Volume
1,0 uL/tube
Mastermix Volume
9,0 uL/tube
Additional Volume
20%
Stock
Final
Reaction Buffer
10 X
1 X
MgCl
2
50,00 mM
1,50 mM
dNTPs
10,00 mM
0,20 mM
Primer 1
10,00 uM
0,50 uM
Primer 2
10,00 uM
0,50 uM
Taq DNA Polymerase
5,0 U/uL
0,05 U/uL
Concentration
Reagents
Reagents
Volume
ddH
2
O
122,88 uL
Reaction Buffer (10 X)
19,20 uL
MgCl2 (50,00 mM)
5,76 uL
dNTPs (10,00 mM)
3,84 uL
Primer 1 (10,00 uM)
9,60 uL
Primer 2 (10,00 uM)
9,60 uL
Taq DNA Polymerase (5,0 U/uL)
1,92 uL
Total Volume
172,80 uL
